Wylie Lab

I have over 20 years experience in large-scale genomics, applying biology, informatics, and analytic skills to a wide range of scientific research. I am highly experienced in the technology development and informatics operations of a large genome center. Early in my career, I contributed to the International Human Genome Sequencing Consortium that sequenced and mapped the first human genome [PMID: 11237011]. Subsequently, I contributed to the sequencing and analysis of other landmark model organisms, including mouse, macaque, platypus, soybean, zebrafish, toxoplasma, and numerous parasitic and free-living nematode species. I contributed to the sequencing and publication of the first human cancer genome [PMID: 18987736], a patient with acute myeloid leukemia, as well as subsequent projects involving sequence data from human cancers. I worked 9 years in the area of technology development at The McDonnell Genome Institute (MGI) under Dr. Elaine R. Mardis, managing a small, agile group of bioinformaticians. This role required being the informatics/analysis “glue” between the development wet lab, bioinformatics team, testing and training, and other collaborative groups at the institute. As such, I have extensive experience handling and analyzing high throughput sequencing data as produced by multiple next-generation sequencing platforms. Prior to joining the Department of Pediatrics, I directed Dr. George Weinstock’s microbial computing group, focusing on software development and analysis for the study of human and microbial genomes. I also have an extensive background in targeted sequence capture, transcriptomics, and the analysis of whole exome data. My colleagues and I recently developed a comprehensive targeted sequence capture panel called ViroCap [PMID: 26395152], designed to enrich nucleic acid from DNA and RNA viruses. This tool will greatly enhance the study of eukaryotic DNA and RNA viruses and takes us closer to using high-throughput sequencing as a comprehensive viral diagnostic tool.

Principal investigator

Todd N. Wylie

Assistant Professor of Pediatrics, Gastroenterology, Hepatology & Nutrition

• Phone: 314-454-6173

Recent publications

• Targeted Metagenomic Sequencing for Detection of Vertebrate Viruses in Wastewater for Public Health Surveillance
  September 8, 2023
  Targeted Metagenomic Sequencing for Detection of Vertebrate Viruses in Wastewater for Public Health SurveillanceMcCall, C., Leo Elworth, R. A., Wylie, K. M., Wylie, T. N., Dyson, K., Doughty, R., Treangen, T. J., Hopkins, L., Ensor, K. & Stadler, L. B., Sep 8 2023, In: ACS ES and T Water. 3, 9, p. 2955-2965 11 p.Research output: Contribution to journal › Article › peer-review
• Metagenomic Analysis of DNA Viruses with Targeted Sequence Capture of Canine Lobular Orbital Adenomas and Normal Conjunctiva
  May 1, 2023
  Metagenomic Analysis of DNA Viruses with Targeted Sequence Capture of Canine Lobular Orbital Adenomas and Normal ConjunctivaSchaefer, E. A. F., Chu, S., Wylie, K. M., Wylie, T. N., Griffith, O. L., Pearce, J. W., Johnson, G. C., Bryan, J. N. & Flesner, B. K., May 2023, In: Microorganisms. 11, 5, 1163.Research output: Contribution to journal › Article › peer-review
• Pathogen and Antibody Identification in Children with Encephalitis in Myanmar
  March 1, 2023
  Pathogen and Antibody Identification in Children with Encephalitis in MyanmarGalardi, M. M., Sowa, G. M., Crockett, C. D., Rudock, R., Smith, A. E., Shwe, E. E., San, T., Linn, K., Aye, A. M. M., Ramachandran, P. S., Zia, M., Wapniarski, A. E., Hawes, I. A., Hlaing, C. S., Kyu, E. H., Thair, C., Mar, Y. Y., Nway, N., Storch, G. A., Wylie, K. M., & 4 othersWylie, T. N., Dalmau, J., Wilson, M. R. & Mar, S. S., Mar 2023, In: Annals of neurology. 93, 3, p. 615-628 14 p.Research output: Contribution to journal › Article › peer-review
• Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
  February 1, 2023
  Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart DiseaseGarand, M., Huang, S. S. Y., Goessling, L. S., Wan, F., Santillan, D. A., Santillan, M. K., Brar, A., Wylie, T. N., Wylie, K. M. & Eghtesady, P., Feb 2023, In: Microorganisms. 11, 2, 262.Research output: Contribution to journal › Article › peer-review
• A Case of Persistent Human Pegivirus Infection in Two Separate Pregnancies of a Woman
  October 1, 2022
  A Case of Persistent Human Pegivirus Infection in Two Separate Pregnancies of a WomanGarand, M., Huang, S. S. Y., Goessling, L. S., Santillan, D. A., Santillan, M. K., Brar, A., Wylie, T. N., Wylie, K. M. & Eghtesady, P., Oct 2022, In: Microorganisms. 10, 10, 1925.Research output: Contribution to journal › Article › peer-review